2-APB

Tocris Bioscience | Catalog # 1224

IP3 receptor antagonist. Also TRP channel modulator
Tocris Bioscience

Key Product Details

Description

IP3 receptor antagonist. Also TRP channel modulator
View all Inositol Lipid Antagonists »

Product Description

2-APB is a functional and membrane permeable D-myo-inositol 1,4,5-trisphosphate (IP3) receptor antagonist (IC50 = 42 μM). Stimulates store-operated calcium (SOC) release at low concentrations (< 10 μM) and inhibits it at higher concentrations (up to 50 μM). Increases STIM-Orai channel conductance and limits ion selectivity. Modulator of TRP channels; blocks TRPC1, TRPC3, TRPC5, TRPC6, TRPV6, TRPM3, TRPM7, TRPM8 and TRPP2 and at higher concentrations stimulates TRPV1, TRPV2 and TRPV3. Also blocks specific gap channel subtypes.

Product Specifications for 2-APB

Molecular Weight

225.10

Formula

C14H16BNO

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

2-Aminoethoxydiphenylborane

CAS Number

524-95-8

PubChem ID

1598

InChI Key

BLZVCIGGICSWIG-UHFFFAOYSA-N

SMILES

NCCOB(C1=CC=CC=C1)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 22.51 100 with gentle warming

Preparing Stock Solutions for 2-APB

The following data is based on the product molecular weight 225.10.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.44 mL 22.21 mL 44.42 mL
5 mM 0.89 mL 4.44 mL 8.88 mL
10 mM 0.44 mL 2.22 mL 4.44 mL
50 mM 0.09 mL 0.44 mL 0.89 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 429 publications citing the usage of this product.

Product Documents for 2-APB

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 2-APB

For research use only

Related Research Areas

Ion Channels and Regulators

Customer Reviews for 2-APB (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 的 4 reviews Showing All
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  • Significantly inhibits TRPM7 current
    Name: Yuyang Sun
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: sh-sy5y
    Verified Customer | Posted 06/29/2022
    It significantly inhibits TRPM7 current. Very good.
    2-APB 1224
  • 2-APB reduces store-operated Ca2+ entry in HEK293 cells
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 07/10/2019
    HEK293 cells were incubated with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min, washed and kept aside for 20 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and fluorescence at 510 nm was expressed as 340/380 ratio. Intracellular Ca2+ stores were depleted with 30 μM BHQ which promotes store-operated Ca2+ entry into the cells upon addition of 1 mM extracellular Ca2+. Preincubation of cells with 50 μM 2-APB for 10 min reduces this Ca2+ entry.
    2-APB 1224
  • IP3R involved or not
    Name: Anonymous
    Assay Type: In Vitro
    Verified Customer | Posted 04/13/2018
    We used 2-APB to determine whether IP3R involved in the related calcium signaling
  • 2-APB in brains slices
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 03/08/2018
    Put it into DMSO (100mM).
    2-APB (50uM) was applied to mouse brain slices while measuring evoked dopamine release.
    2-APB 1224

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