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5(6)-SFX (Fluorescein), SE

Catalog #: 6488 Datasheet / COA / SDS

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6488 has been discontinued.
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5(6)-SFX (Fluorescein), SE | CAS No. 114616-31-8 | Standard Fluorescein, Coumarin and Rhodamine-based
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Description: Green fluorescent dye for the labeling of primary amines

Chemical Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

Purity: ≥90%

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Biological Activity

5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Optical Data for 5(6)-SFX (Fluorescein), SE

Emission Color Green
λabs 494 nm
λem 518 nm
Extinction Coefficient (ε) 70000 M-1cm-1
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Application Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
586.55
Formula:
C31H26N2O10
Solubility:
Soluble to 100 mM in DMSO and to 100 mM in DMF
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
114616-31-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Artificial human Met agonists based on macrocycle scaffolds.
    Ito, Kenichir, Sakai, Katsuya, Suzuki, Yoshinor, Ozawa, Naoya, Hatta, Tomohisa, Natsume, Tohru, Matsumoto, Kunio, Suga, Hiroaki
    Nat Commun, 2015-03-11;6(0):6373.

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