5-Azacytidine

Key Product Details

Product Description

5-Azacytidine is a DNA methyltransferase inhibitor. Incorporates into DNA forming covalent adducts with cellular DNMT1, depleting enzyme activity. Induces demethylation and reactivation of silenced genes. Improves the efficiency of reprogramming of stem cells; induces differentiation of mesenchymal stem cells into cardiomyocytes.

Product Specifications for 5-Azacytidine

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for 5-Azacytidine

The following data is based on the product molecular weight 244.20.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

4 3 2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.10 mL	20.48 mL	40.95 mL
5 mM	0.82 mL	4.10 mL	8.19 mL
10 mM	0.41 mL	2.05 mL	4.10 mL
50 mM	0.08 mL	0.41 mL	0.82 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 13 publications citing the usage of this product.

• Mikkelsen Dissecting direct reprogramming through integrative genomic analysis. Nature 2008 PMID: 18509334
• Qian 5-Azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated kinase. Stem Cells Dev. 2012 PMID: 21476855
• Schneider-Stock 5-aza-Cytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms. J.Pharmacol.Exp.Ther. 2005 PMID: 15547111

Product Documents for 5-Azacytidine