Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

Novus Biologicals | Catalog # NBP3-33135

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE63-95 expressed in HEK293
View all Acetyl-CoA Carboxylase alpha/ACACA Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser79 of Mouse Acetyl-CoA Carboxylase alpha/ACACA protein. (Uniprot: Q13085)

Modification

p Ser 79

Localization

Cytoplasm, cytosol.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

265 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33135) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 265 kDa
Observed band size: 265 kDa

Exposure time: 1 minutes 14 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33135) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acetyl-CoA Carboxylase alpha/ACACA

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Alternate Names

ACACA, ACC1, ACCA, AcetylCoA Carboxylase alpha

Gene Symbol

ACACA

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Product Documents for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

Certificate of Analysis

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Product Specific Notices for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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