AMICA/JAML Antibody - BSA Free

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• AMICA/JAML Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Paraffin

  Sample Tested: kidney

  Species: Mouse

  Verified Customer | Posted 12/15/2016

  Antibody Storage Conditions:Store at 4C short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles. Test Sample Information: Species & Treatments:mouse Tissue: kidney Fixative Composition: 4% Paraformaldehyde solution Fixation Time & Temperature: 24h，4℃ Tissue Processing: paraffin embedding Antigen Retrieval Details (for IHC-P): Method & Buffer Used: Citric Acid Antigen Retrieval Buffer Time & Temperature: >95℃ 20min Blocking Procedure: Blocking Solution: 5% Goat serum，0.1%BSA Time & Temperature: 1h，room temperature Endogenous Peroxidase Blocking (For IHC-P only) H2O2 cocktail composition: 3% H2O2 Blocking Time: 10min Primary Antibody Dilution: 1:100 Diluent Buffer: 0.1%BSA（PBS） Time & Temperature: overnight，4℃ Washing Conditions: Wash Buffer Composition: PBS Method (Immersion, Rinsing etc.): Immersion Times/Washing: 5min Repetitions: 3 Secondary Antibody Manufacturer and Catalog #: Zhongshan golden bridge ZB-2301 Secondary description:goat anti-rabbit Dilution: 1:100 Diluent Buffer: PBS Incubation Time & Temperature: 1h，room temperature Post-Secondary Washing: 5min X 3 times Detection Method: Detection: Zhongshan golden bridge SP-9000 Procedure: according to the instruction book DAB-Incubation Time (if applicable):3min Controls: Negative Control: Control rabbit IgG

  

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