Anti-Daclizumab (Anti-Idiotype) Antibody (2498A)

R&D Systems | Catalog # MAB10218

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2498A
View all Daclizumab Primary Antibodies »

Product Specifications

Immunogen

Full length Daclizumab

Specificity

Detects Daclizumab in direct ELISAs and Western blots. In Western blots, no cross-reactivity with Basiliximab, Satumomab, Teplizumab, and Alemtuzumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti-Daclizumab (Anti-Idiotype) Antibody (2498A)

Detection of Daclizumab antibody by Western Blot.

Detection of Daclizumab by Western Blot.

Western blot shows Daclizumab, Basiliximab (negative control), Satumomab (negative control), Teplizumab (negative control), and Alemtuzumab (negative control) biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Daclizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10218) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Daclizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Anti-Daclizumab (Anti-Idiotype) Antibody (2498A)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Daclizumab
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Daclizumab

The anti-Daclizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Daclizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Daclizumab Products

Product Documents for Anti-Daclizumab (Anti-Idiotype) Antibody (2498A)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Anti-Daclizumab (Anti-Idiotype) Antibody (2498A)

For research use only

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FAQs for Anti-Daclizumab (Anti-Idiotype) Antibody (2498A)

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  • Q: What is an anti-idiotype antibody?

    A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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