API-2

Tocris Bioscience | Catalog # 2151

Selective inhibitor of Akt/PKB signaling. Antitumor and antiviral
Tocris Bioscience

Key Product Details

Description

Selective inhibitor of Akt/PKB signaling. Antitumor and antiviral

Alternative Names

Triciribine,NSC 154020,TCN
View all Akt (Protein Kinase B) Inhibitors »

Product Description

API-2 is a selective inhibitor of Akt (protein kinase B) signaling; displays minimal inhibition of PKC, PKA, SGK and p38 pathways. Inhibits phosphorylation and activation of downstream targets of Akt including Bad, GSK-3β and AFX. Induces apoptosis and growth arrest in vitro, preferentially in human cancer cells with elevated levels of Akt. Potently and selectively inhibits growth of Akt-overexpressing tumors in mice. Inhibits DNA synthesis and displays antiviral activity against HIV-1 and -2.

Product Specifications for API-2

Molecular Weight

320.31

Formula

C13H16N6O4

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

1,5-Dihydro-5-methyl-1-β-D-ribofuranosyl-1,4,5,6,8-pentaazaacenaphthylen-3-amine

CAS Number

35943-35-2

PubChem ID

65399

InChI Key

HOGVTUZUJGHKPL-HTVVRFAVSA-N

SMILES

O[C@@H]1[C@@H](CO)O[C@@H](N2C3=C(C4=NC=N3)C(C(N)=NN4C)=C2)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1eq. HCl 32.03 100
DMSO 32.03 100

Preparing Stock Solutions for API-2

The following data is based on the product molecular weight 320.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.22 mL
5 mM 0.62 mL 3.12 mL 6.24 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 46 publications citing the usage of this product.

Product Documents for API-2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for API-2

For research use only

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  • Attenuation by co-treatment of either PI3K inhibitor LY94002 or Akt inhibitor API-2
    Name: Zhenmin Lei
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Ovarian theca cells
    Verified Customer | Posted 01/04/2018
    The isolated mouse ovarian theca cells were maintained in 12-well plates with RPMI-1640 medium. For determination of Lhcgr expression, the cells were rinsed three times with PBS the day before incubation with vehicle (DMSO), 740YP (50 mg/ml), 740YP + API-2 (7.5 mM), LH (50 ng/mL), or LH + API-2 for 24 h. For determination of Akt phosphorylation, PBS rinsed cells were incubated with vehicle (DMSO), or API-2 (7.5 mM) in serum-free RPMI-medium for 1 h before the addition of LH (50 ng/mL) for 30 min.
    API-2 2151

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