Recombinant Mouse APRIL/TNFSF13 Protein

Novus Biologicals | Catalog # NBP2-35145

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
View all APRIL/TNFSF13 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to APRIL/TNFSF13 Source: E. coli

Uniprot ID: Q9D777

Amino Acid Sequence: AVLTQKHKKK HSVLHLVPVN ITSKADSDVT EVMWQPVLRR GRGLEAQGDI VRVWDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIRSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of APRIL/TNFSF13 as determined by LAL method.

Predicted Molecular Mass

16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TNFSF13 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using activated T cells.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-35145
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APRIL/TNFSF13

A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice. Recombinant murine soluble APRIL is a 16.4 kDa protein, consisting of 146 amino acid residues.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2, sAPRIL, sAPRIL/TNFSF13

Gene Symbol

TNFSF13

Additional APRIL/TNFSF13 Products

Product Documents for Recombinant Mouse APRIL/TNFSF13 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse APRIL/TNFSF13 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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