Aquaporin-3 Overexpression Lysate
Novus Biologicals | Catalog # NBL1-07638
![Western Blot: Aquaporin-3 Overexpression Lysate [NBL1-07638]](https://resources.rndsystems.com/images/products/Aquaporin-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07638-img0002.jpg "Western Blot: Aquaporin-3 Overexpression Lysate [NBL1-07638]")
Key Product Details
Species
Applications
Product Summary for Aquaporin-3 Overexpression Lysate
Aquaporin-3 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC201856
Accession#: NM_004925
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for Aquaporin-3 Overexpression Lysate
Western Blot: Aquaporin-3 Overexpression Lysate [NBL1-07638]
Western Blot: Aquaporin-3 Overexpression Lysate (Adult Normal) [NBL1-07638] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aquaporin-3.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: Aquaporin-3
Alternate Names
Gene Symbol
Additional Aquaporin-3 Products
Product Documents for Aquaporin-3 Overexpression Lysate
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Aquaporin-3 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Aquaporin-3 Overexpression Lysate
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Q: One of our customer ordered antibody to aquaporin 3 cat# NBP1-46502. She doesn't manage to make it work in WB so she wanted to know if you can advise on what molecular might she can except to see the band? She also wanted to order the positive control lysate NBL1-07638 but there are so many bands on the blot that she's afraid it won't help! Could you tell me which antibody was used on the blot picture we can see on the web? And what dilution was used? Could you just confirm if the image from NBL1-07638 datasheet was obtained with antibody NBP1-46502 or another antibody? Then as there are so many bands, would you be able to supply a blocking peptide to switch off the specific band? Which kind of washing buffer and blocking buffer do you suggest? Which steps (how many and how long would you suggest)?
A: The Theoretical MW of AQP3 is 32 KDa. This antibody is unpurified and therefore may detect more unspecific bands than a purified antibody. I would recommend increasing the blocking and washing times to alleviate this issue, along with diluting the antibody further. NBP1-64502 was not used on NBL1-07638, and while it should work I would recommend trying the suggestions above first. Unfortunately we don't have a blocking peptide available for this product.