ASAP1 Antibody

Novus Biologicals | Catalog # NBP2-24643

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ASAP1 Primary Antibodies »

Product Specifications

Immunogen

Within the range of amino acids 962-999 of mouse ASAP1 were used as the immunogen.

Reactivity Notes

Chicken (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASAP1 Antibody

Western Blot: ASAP1 Antibody [NBP2-24643]

Western Blot: ASAP1 Antibody [NBP2-24643]

Western Blot: ASAP1 Antibody [NBP2-24643] - Analysis of ASAP1 in mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24643 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for ASAP1 Antibody

Application
Recommended Usage

Western Blot

2.0-3.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASAP1

ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes. Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.

Alternate Names

AMAP1, CENTB4, DDEF1, PAG2, ZG14P

Gene Symbol

ASAP1

Additional ASAP1 Products

Product Documents for ASAP1 Antibody

Certificate of Analysis

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Product Specific Notices for ASAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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