ASP 7967

Tocris Bioscience | Catalog # 8803

Ligand for RNA aptamer AC17-4 activating gene expression in aptazime-based riboswitches
Tocris Bioscience

Key Product Details

Description

Ligand for RNA aptamer AC17-4 activating gene expression in aptazime-based riboswitches
View all DNA, RNA and Protein Synthesis Compounds »

Product Description

ASP 7967 is a ligand for RNA aptamer AC17-4 that activates gene expression in synthetic aptazime-based riboswitches at as low as 5 μM concentration, without relying on exogenous protein factors. ASP 7967 binds aptamer AC17-4 with high affinity in HEK293 cells (Kd = 12 nM; EC50 = 2.3 μM), activating gene expression (>10-fold) in HEK293 cells. ON/OFF ratio of riboswitches activated by ASP 7967 approaches 300, with a low basal expression level in cultured cells.

In vivo, ASP 7967 upregulates the serum hEPO concentration by 7.2-fold over the vehicle control (mice). Cell permeable and low-toxic, ASP 7967 is orally bioavailable.

Product Specifications for ASP 7967

Molecular Weight

464.44

Formula

C20H16F2N8.½C4H4O4

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

N2,N4-Bis(4-fluorophenyl)-N6-(2-pyrimidinylmethyl)-1,3,5-triazine-2,4,6-triamine hemifumarate

CAS Number

1246958-42-8

PubChem ID

46931459

InChI Key

PQVNSJGSJVAEAC-WXXKFALUSA-N

SMILES

FC1=CC=C(C=C1)NC2=NC(NCC3=NC=CC=N3)=NC(NC4=CC=C(F)C=C4)=N2.OC(/C=C/C(O)=O)=O.FC5=CC=C(C=C5)NC6=NC(NCC7=NC=CC=N7)=NC(NC8=CC=C(F)C=C8)=N6

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 46.44 100

Preparing Stock Solutions for ASP 7967

The following data is based on the product molecular weight 464.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.15 mL 10.77 mL 21.53 mL
5 mM 0.43 mL 2.15 mL 4.31 mL
10 mM 0.22 mL 1.08 mL 2.15 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for ASP 7967

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ASP 7967

For research use only

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