BAP28 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92105

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all BAP28 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HEATR1 (NP_060542.4). MTSLAQQLQRLALPQSDASLLSRDEVASLLFDPKEAATIDRDTAFAIGCTGLEELLGIDPSFEQFEAPLFSQLAKTLERSVQTKAVNKQLDENISLFLIHLSPYFLLKPAQKCLEWLIHR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

242 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAP28 Antibody - Azide and BSA Free

Western Blot: BAP28 AntibodyAzide and BSA Free [NBP2-92105]

Western Blot: BAP28 AntibodyAzide and BSA Free [NBP2-92105]

Western Blot: BAP28 Antibody [NBP2-92105] - Analysis of extracts of HeLa cells, using BAP28 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time:

Applications for BAP28 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAP28

IRAK-4 is a member of the interleukine-1 receptor-associated kinase (IRAK) family. The IRAK family is implicated in the Toll-like receptor (TLR) and Il-1R signaling pathway (1). Upon phosphorylation, IRAK-4 interacts with IRAK-1, MYD88 and TRAF6 to form a complex, and activate NF-kB and MAPK pathways (2-3). IRAK-4 phosphorylates and activates IRAK-1 at thr387 and is also known to interact with IRAK-1 but not with the other IRAK members. Additionally, like IRAK-1, IRAK-4 possesses an auto and cross-phosphorylation kinase activity (4).

Alternate Names

BAP28HEAT repeat-containing protein 1, EC 4.1.1.31, EC 6.3.4.4, FLJ10359, HEAT repeat containing 1, MGC72083, Protein BAP28

Gene Symbol

HEATR1

Additional BAP28 Products

Product Documents for BAP28 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BAP28 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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