BDY 630-X, SE
Tocris Bioscience | Catalog # 5468
Key Product Details
Description
Wavelength
Product Description
BDY 630-X, SE is a fluorescent red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 630-X makes it ideal for labeling lipid and cell membranes. BDY 630-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~625 nM; emission maximum ~640 nM.
For more information on how BDY 630-X, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data
| Emission Color | Red |
| λabs | 625 nm |
| λem | 640 nm |
| Extinction Coefficient (ε) | 101000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.029 |
| Correction Factor 280 | 0.035 |
| Application | Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | BDY 650-X, SE; Cy5; Alexa Fluor 647 |
Spectra Viewer
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Product Specifications for BDY 630-X, SE
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 66.05 | 100 |
Preparing Stock Solutions for BDY 630-X, SE
The following data is based on the product molecular weight 660.50.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.51 mL | 7.57 mL | 15.14 mL |
| 5 mM | 0.30 mL | 1.51 mL | 3.03 mL |
| 10 mM | 0.15 mL | 0.76 mL | 1.51 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.30 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Vernall Highly potent and selective fluorescent antagonists of the human adenosine A3 receptor based on the 1,2,4-triazolo[4,3-a]quinoxalin-1-one scaffold. J.Med.Chem. 2012 PMID: 22277057
Product Documents for BDY 630-X, SE
Product Specific Notices for BDY 630-X, SE
For research use only
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