Biotin BromoCatch™ Ligand

Tocris Bioscience | Catalog # 8939

A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
Tocris Bioscience

Key Product Details

Description

A biotinylated covalent ligand for BromoCatch-tagged proteins, enabling selective and irreversible biotin labeling for pull-down and detection applications in cell lysates.
View all BromoCatch Platform »

Product Description

Biotin BromoCatch™ Ligand is a covalent probe designed for the selective labeling and enrichment of proteins tagged with the BromoCatch™ self-labeling tag. Based on a para-acrylamide-functionalized bump-JQ1 scaffold, the BromoCatch™ ligand, enables irreversible and highly specific covalent attachment to the engineered cysteine residue in the BromoCatch-tagged protein. The appended biotin handle allows for efficient affinity-based detection, pull-down, and isolation of tagged proteins from complex mixtures such as cell lysates. This probe demonstrates exceptional target selectivity, confirmed via intact protein mass spectrometry and western blotting, and shows no background labeling in untransfected or wild-type samples even at micromolar concentrations. Biotin BromoCatch™ Ligand expands the utility of the BromoCatch™ platform for biochemical workflows including proteomics, immunoprecipitation, and interaction studies.

Please refer to this protocol for further information on how to use this product.

This product is part of an early adopter program.

BromoCatch™ is a trademark of Bio-Techne Corp.

Licensing Information

Sold under exclusive licence from the University of Dundee

Product Specifications for Biotin BromoCatch™ Ligand

Molecular Weight

908.15

Formula

C44H61N9O8S2

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-((17R)-17-((6S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-16-oxo-3,6,9,12-tetraoxa-15-azanonadecyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

InChI Key

BZQKBFKRTBEEFV-HQANLXDBSA-N

SMILES

CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCOCCNC(CCCC[C@H]4[C@](NC(N5)=O)([H])[C@]5([H])CS4)=O)=O)CC)C6=NN=C(C)N26

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product.

Product Documents for Biotin BromoCatch™ Ligand

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Biotin BromoCatch™ Ligand

For research use only

Customer Reviews for Biotin BromoCatch™ Ligand

There are currently no reviews for this product. Be the first to review Biotin BromoCatch™ Ligand and earn rewards!

Have you used Biotin BromoCatch™ Ligand?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card