Biotin Check&Go! Control Kit

Novus Biologicals | Catalog # 4001-0030

Novus Biologicals
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Key Product Details

Kit Type

Control Kit

Product Summary for Biotin Check&Go! Control Kit

Biotin Check&Go! allows scientists to confirm the success of their antibody biotinylation in one easy step.

Unlike traditional methods of assessing the success of biotinylation, such as the HABA assay, which can be tedious and time consuming, Biotin Check&Go! is a simple immunochromatography test that requires only 10 minutes of your time and very small quantities of your antibody conjugate.

The key components of the kit include a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G, both which have a high affinity for the Fc region of a variety of IgG molecules, and a 40nm InnovaCoat GOLD Streptavidin conjugate for detection.

  • Quickly check the success of your conjugation (only 10 minutes!)
  • Only takes 10 minutes!
  • No costly equipment required
  • Compatible with antibody conjugates generated using our Lightning-Link Biotin antibody labeling kits and other biotin labeling technologies available commercially
Requires only small volumes (20ul of diluted conjugate)Unique to Expedeon
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Product Specifications

Application Notes

This product is suitable for use with Lightning-Link Biotin antibody labeling kits as well as other biotinylation technologies.

Scientific Data Images for Biotin Check&Go! Control Kit

Antibody Labeling Kit [4001-0030] - When the biotinylated antibody is mixed with the streptavidin-gold conjugate it forms a complex that flows along the nitrocellulose membrane. Upon binding to the Protein A and Protein G concentrated on the Test line, a visible red line appears on the strip.

Kit Contents for Biotin Check&Go! Control Kit

  • 1 vial of 40 nm Innova Coat® GOLD Streptavidin
  • 1 vial of Positive Control
  • 2 cryovials of 10x Running Buffer
  • 30 Protein A/G Strips
  • Not supplied: 96-wells low binding plate Bovine serum albumin (BSA)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Product Documents for Biotin Check&Go! Control Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Biotin Check&Go! Control Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Biotin Check&Go! Control Kit

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FAQs

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