Bis-ANS

Biological Activity

Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect Aβ fibre (K_d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase separation (LLPS) of TDP-43 low complexitry domain in vitro. Displays biphasic modulation; promotes LLPS at low concentrations but disprut liquid droplets at higher concentrations.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Dimer formation from 1-amino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties.
   Rosen et al.
   Biochemistry, 1969;8:3915
2. A comparison of three fluorophores for the detection of amyloid fibers and prefibrillar oligomeric assemblies. ThT (Thioflavin T); ANS (1-Anilinonaphthalene-8-sulfonic Acid); and bisANS (4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid).
   Younan et al.
   Biochemistry, 2015;54:4297
3. Small molecules as potent biphasic modulators of protein liquid-liquid phase separation
   Babinchak et al.
   Nat.Commun., 2020;11:5574

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