Bis-ANS

Key Product Details

Product Description

Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect Aβ fibre (K_d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase separation (LLPS) of TDP-43 low complexitry domain in vitro. Displays biphasic modulation; promotes LLPS at low concentrations but disprut liquid droplets at higher concentrations.

Product Specifications for Bis-ANS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Bis-ANS

The following data is based on the product molecular weight 672.85.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.49 mL	7.43 mL	14.86 mL
5 mM	0.30 mL	1.49 mL	2.97 mL
10 mM	0.15 mL	0.74 mL	1.49 mL
50 mM	0.03 mL	0.15 mL	0.30 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Younan A comparison of three fluorophores for the detection of amyloid fibers and prefibrillar oligomeric assemblies. ThT (Thioflavin T); ANS (1-Anilinonaphthalene-8-sulfonic Acid); and bisANS (4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid). Biochemistry 2015 PMID: 26087242
• Babinchak Small molecules as potent biphasic modulators of protein liquid-liquid phase separation Nat.Commun. 2020 PMID: 33149109
• Rosen Dimer formation from 1-amino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties. Biochemistry 1969 PMID: 5388144

Product Documents for Bis-ANS