Blooms Syndrome Protein Blm Antibody - BSA Free

Western Blot: Blooms Syndrome Protein Blm Antibody - BSA Free [NB100-214] -

TRF2 phosphorylation promotes BTR-dependent MAD telomere deprotection.a BLM domain structure and the mutant alleles used in this study. The compromised function of the mutant alleles is indicated. b, c Above, Metaphase-telomere deprotection induced foci (TIF) following 24 h of 100 ng mL−1 colcemid in IMR90 E6E7 hTERT expressing Control or BLM shRNA and shRNA-resistant BLM alleles (all data points from n = 3 biological replicates of 30 metaphases per replicate, compiled into a Tukey Box Plot, Kruskal-Wallis followed by Dunn’s multiple comparisons test). Below: representative immunoblots of whole cell extracts derived from the untreated cell cultures used in this experiment. d TOP3A domain structure and the mutant allele used in this study. e Above, Metaphase-TIF following 24 h of 100 ng mL−1 colcemid in IMR90 E6E7 hTERT fibroblasts expressing Control or TOP3A shRNA and vector or shRNA-resistant TOP3A alleles (all data points from n = 3 biological replicates of 30 metaphases per replicate compiled into a Tukey Box Plot, Kruskal-Wallis followed by Dunn’s multiple comparisons test). Below, representative immunoblots of whole cell extracts derived from the untreated cell cultures used in this experiment. f Above, Metaphase-TIF following 24 h of 100 ng mL−1 colcemid in IMR90 E6E7 hTERT expressing Control, BLM or TRF2 shRNA, and vector or TRF2-2D (S62D and S65D) (all data points from n = 3 biological replicates of 30 metaphases per replicate compiled into a Tukey Box Plot, Kruskal-Wallis followed by Dunn’s multiple comparisons test). Below, representative immunoblots of whole cell extracts derived from the untreated cell cultures used in this experiment. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40097392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.