Bovine SPAM1 Antibody

Name: Bovine SPAM1 Antibody
Brand: R&D Systems
SKU: MAB6436

Catalog #: MAB6436 Datasheet / COA / SDS

Discontinued Product

MAB6436 has been discontinued.

View all SPAM1 products.

Detection of Bovine SPAM1 by Western Blot.

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All SPAM1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Bovine SPAM1 Antibody Summary

Species Reactivity

Bovine

Specificity

Detects bovine SPAM1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 763056

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant bovine SPAM1
Met1-Thr497
Accession # AAI10184

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Bovine SPAM1 by Western Blot. Western blot shows Recombinant Bovine SPAM1 (Catalog # 6436-GH) (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine SPAM1 Monoclonal Antibody (Catalog # MAB6436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPAM1

Sperm adhesion molecule 1 (SPAM1), also known as PH-20 hyaluronidase (1), is encoded by one of the six hyaluronidase-like genes (1-3). SPAM1 is a GPI‑anchored enzyme located on the sperm surface and inner acrosomal membrane (4). SPAM1 degrades hyaluronic acid (HA), a major structural glycosaminoglycan found in extracellular matrices and basement membranes. The enzyme activity enables sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte and therefore facilitates the fertilization process (5-7). Because of the structural similarity between HA (repeating units of GlcA beta 1-3GlcNAc) and chondroitin sulfate (repeating units of GlcA beta 1-3GalNAc), the enzyme is also able to hydrolyze chondroitin sulfate. The bovine SPAM1 is 63% identical to human homologue in sequence.

References

Lathrop, W.F. et al. (1990) J. Cell Biol. 111:2939.
Lin, Y. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:10071
Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
Phelps, B.M. et al (1988) Science 240:4860
Gmachl, M. et al. (1993) FEBS Lett. 336:545.
Hofinger, E.S et al. (2007) Glycobiology 17:963.
Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11. 8.

Long Name

Sperm Adhesion Molecule 1

Entrez Gene IDs

6677 (Human); 353352 (Bovine)

Alternate Names

EC 3.2.1.35; HYA1; HYAL1; HYAL3; HYAL5; hyal-PH20; hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; MGC26532; PH20; PH-20; SPAG15; SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); Sperm adhesion molecule 1; Sperm surface protein PH-20