BRD 0539

Tocris Bioscience | Catalog # 7153

Cell permeable and reversible Cas9 inhibitor
Tocris Bioscience

Key Product Details

Description

Cell permeable and reversible Cas9 inhibitor
View all CRISPR Reagents »

Product Description

BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC50 = 22 μM) and in an eGFP disruption assay (apparent EC50 = 11 μM). Allows dose and temporal control of SpCas9-based systems.

Licensing Information

Sold under licence from the Broad Institute

Product Specifications for BRD 0539

Molecular Weight

452.54

Formula

C25H25FN2O3S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(3aR,4R,9bR)-8-(2-Fluorophenyl)-2,3,3a,4,5,9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3,2-c]quinoline-4-methanol

CAS Number

1403838-79-8

PubChem ID

54666326

InChI Key

CZOIXFMISSBIJL-DCEDVJGZSA-N

SMILES

[H][C@]12CCN([C@@]1([H])C1=CC(=CC=C1N[C@H]2CO)C1=C(F)C=CC=C1)[S](=O)(=O)C1=CC=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 45.25 100
Ethanol 45.25 100

Preparing Stock Solutions for BRD 0539

The following data is based on the product molecular weight 452.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.21 mL 11.05 mL 22.10 mL
5 mM 0.44 mL 2.21 mL 4.42 mL
10 mM 0.22 mL 1.10 mL 2.21 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for BRD 0539

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BRD 0539

For research use only

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