Caffeine

Tocris Bioscience | Catalog # 2793

A1 and A2B antagonist. CNS stimulant
Tocris Bioscience

Key Product Details

Description

A1 and A2B antagonist. CNS stimulant
View all Non-selective Adenosine Receptor Antagonists »

Product Description

Caffeine is a central nervous system stimulant. Antagonist at A1 and A2A adenosine receptors and inhibitor of cyclic nucleotide phosphodiesterases. Mobilizes calcium from intracellular stores and inhibits benzodiazepine binding to GABA receptors.

Product Specifications for Caffeine

Molecular Weight

194.19

Formula

C8H10N4O2

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione

CAS Number

58-08-2

PubChem ID

2519

InChI Key

RYYVLZVUVIJVGH-UHFFFAOYSA-N

SMILES

O=C1C2=C(N=CN2C)N(C)C(N1C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 19.42 100
DMSO 9.71 50

Preparing Stock Solutions for Caffeine

The following data is based on the product molecular weight 194.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.15 mL 25.75 mL 51.50 mL
5 mM 1.03 mL 5.15 mL 10.30 mL
10 mM 0.51 mL 2.57 mL 5.15 mL
50 mM 0.10 mL 0.51 mL 1.03 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 38 publications citing the usage of this product.

Product Documents for Caffeine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Caffeine

For research use only

Customer Reviews for Caffeine (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Caffeine?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 的 3 reviews Showing All
Filter By:
Clear Filters X
  • Low-dose oral caffeine induces a specific form of behavioral sensitization
    Name: Kevin Ball
    Species: Rat
    Assay Type: In Vivo
    Verified Customer | Posted 01/28/2019
    Rats had free access to untreated water or water containing 0.2 mg/ml of caffeine every other day of a 14-day experiment. This dose is relevant to human caffeine intake. Rats were tested on alternated days in an open-field arena for discrete movements (horizontal and vertical) and ambulatory distance. Results showed that although caffeine intake decreased significantly across test sessions, the number of discrete horizontal movements increased significantly, that is, showed sensitization.
    Caffeine 2793
  • Caffeine acts on RyR2 in HL1 cells to release ER Ca2+.
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: HL-1 Atrial Muscle Cell Line
    Verified Customer | Posted 12/16/2018
    HL-1 cells were loaded with cytosolic Ca2+ sensitive dye, Fura-2AM (1 µM)+F127 (0.01%) in normal tyrode solution for 40 min and excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm. When the cells are perfused with 10 mM caffeine in nominally Ca2+ free tyrode solution, caffeine acts on RyR2 to release ER Ca2+, resulting in transient cytosolic peak (shown in the figure).
    Caffeine 2793
  • Caffeine as a tool to estimate sarcoplasmic Ca2+ content in cardiomyocytes
    Name: Christopher Trummer
    Species: Guinea Pig
    Assay Type: In Vitro
    Cell Line/Tissue: primary ventricular cardiomyocytes
    Verified Customer | Posted 10/15/2018
    Control or saxagliptin treated primary guinea pig cardiomyocytes were loaded with FURA-2AM (1 µM) and Pluronic F 127 (1%). Ca2+ transient decay tau evoked by caffeine (30 µM) is shown in the Image. Caffeine acts as a ryanodine receptor agonist and releases Ca2+ from sarcoplasmic reticulum into the cytosol.
    Caffeine 2793

There are no reviews that match your criteria.

Showing  1 - 3 的 3 reviews Showing All