Calcein AM

Tocris Bioscience | Catalog # 5119

Cell permeable non-fluorescent compound; green-fluorescent in living cells once hydrolyzed. Used for: cell tracing and cell viability monitoring. Application: fluorescent microscopy and flow cytometry
Tocris Bioscience

Key Product Details

Description

Cell permeable non-fluorescent compound; green-fluorescent in living cells once hydrolyzed. Used for: cell tracing and cell viability monitoring. Application: fluorescent microscopy and flow cytometry

Wavelength

Excitation = 495 nm, Emission = 515 nm
View all Cell Viability Stains and Dyes »

Product Description

Key information: Calcein AM is a cell permeable non-fluorescent compound, that becomes green fluorescent once hydrolyzed in live cells.

Used for: cell tracing, monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.

Application: fluorescent microscopy and flow cytometry.

Properties and Photophysical Data: in live cells, non-fluorescent Calcein AM is hydrolyzed by intracellular esterases into green-fluorescent calcein, which is retained in the cytoplasm. Excitation and emission maxima (λ) are 495 nm and 515 nm, respectively.

It is recommended to prepare stock solutions of Calcein AM in DMSO.

Optical Data

Calcein AM Dye Spectra
Emission Color Green
λabs 495 nm
λem 515 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescent Microscopy

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for Calcein AM

Molecular Weight

994.86

Formula

C46H46N2O23

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester

CAS Number

890090-35-4

PubChem ID

4126474

InChI Key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

SMILES

O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 20 publications citing the usage of this product.

Product Documents for Calcein AM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Calcein AM

For research use only

Customer Reviews for Calcein AM (4)

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  • Works well
    Name: Krish K
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: Spinal neurons
    Verified Customer | Posted 09/11/2025
    To measure toxicity in rat spinal cord neurons
    Calcein AM 5119
  • MCF7 Spheroids viability
    Name: Caterina Suelzu
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: MCF7
    Verified Customer | Posted 11/26/2024
    MCF7 cells spheroids were stained with 1 uM Calcein AM for 1.5h
    Calcein AM 5119
  • HCT116 spheroids
    Name: Gabriele Strusi
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HCT116
    Verified Customer | Posted 11/26/2024
    Viability staining of HCT116 spheroids 1 uM concentration
    Calcein AM 5119
  • cell viability assay
    Name: Karthik krishnamurthy
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: motor neurons
    Verified Customer | Posted 10/24/2018
    Neurons were treated with 50 uM glutamate for 1 hour. To assess the neuronal viability, neurons were incubated with calcein-AM (2 uM), and ethidium homodimer (4 uM) in HEPES buffer for 45-60 min at 37 degrees, rinsed with HEPES 4-5 times and examined under an epifluorescence microscope. This assay clearly distinguished live cells stained positive for calcein (green) from the dead cells stained positive for ethidium homodimer (red).
    For analyzing cellular toxicity in neurons
    Calcein AM 5119

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