Caldesmon/CALD1 Antibody (6M5Q9)

Novus Biologicals | Catalog # NBP3-16499

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6M5Q9 expressed in HEK293
View all Caldesmon/CALD1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 694-793 of human Caldesmon/CALD1 (Q05682). PTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTSPTKV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caldesmon/CALD1 Antibody (6M5Q9)

Western Blot: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499]

Western Blot: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499]

Western Blot: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499] - Western blot analysis of extracts of HeLa cells, using Caldesmon/CALD1 antibody (NBP3-16499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499] - Immunohistochemistry of paraffin-embedded human placenta using Caldesmon/CALD1 Rabbit mAb (NBP3-16499) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (6M5Q9) [NBP3-16499] - Immunohistochemistry of paraffin-embedded rat ovary using Caldesmon/CALD1 Rabbit mAb (NBP3-16499) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Caldesmon/CALD1 Antibody (6M5Q9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody (6M5Q9)

Certificate of Analysis

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Product Specific Notices for Caldesmon/CALD1 Antibody (6M5Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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