Carbonic Anhydrase VIII/CA8 Antibody (005) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90710

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 005 expressed in HEK293

Format

Azide and BSA Free
View all Carbonic Anhydrase VIII/CA8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII/CA8 (Uniprot#: P28651; Met1-Gln291).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase VIII/CA8 Antibody (005) - Azide and BSA Free

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Anti-Carbonic Anhydrase VIII/CA8 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa
Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]

Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]

Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDa

Applications for Carbonic Anhydrase VIII/CA8 Antibody (005) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase VIII/CA8

Carbonic anhydrase-related protein (CARP) is a novel IP3R1-binding protein. Analysis of this protein revealed it is expressed exclusively in the Purkinje cells of the cerebellum.

Alternate Names

CA8, CALS, CARP

Gene Symbol

CA8

Additional Carbonic Anhydrase VIII/CA8 Products

Product Documents for Carbonic Anhydrase VIII/CA8 Antibody (005) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Carbonic Anhydrase VIII/CA8 Antibody (005) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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