Carboxymethyl Lysine Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB3247T Datasheet / COA / SDS

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FAB3247T-100UG

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Carboxymethyl Lysine Alexa Fluor® 594-conjugated Antibody Summary

Specificity

Detects Carboxymethyl Lysine adducts on proteins in Western blots. In Western blots, no cross-reactivity with carboxyethyl lysine adducts is observed.

Source

Monoclonal Mouse IgG2b Clone # 318003

Purification

Protein A or G purified

Immunogen

Carboxymethyl Lysine-modified Keyhole Limpet Hemocyanin (KLH)

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carboxymethyl Lysine

N epsilon -carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.

Alternate Names

Carboxymethyl Lysine; CML