Caspase 3/7 Activity Assay Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-50230
![Caspase 3/7 Activity Assay Kit (Colorimetric) Application: Caspase 3/7 Activity Assay Kit (Colorimetric) [NBP3-50230]](https://resources.rndsystems.com/images/products/nbp3-50230_-elisa-639215491813774662.png "Application: Caspase 3/7 Activity Assay Kit (Colorimetric) [NBP3-50230]")
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Key Product Details
Sample Type & Volume Required Per Well
Cell and tissue samples
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Product Specifications
Kit Type
Activity Assay Kit (Colorimetric)
Species
Non-species specific
Description
Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.
Scientific Data Images for Caspase 3/7 Activity Assay Kit (Colorimetric)
Application: Caspase 3/7 Activity Assay Kit (Colorimetric) [NBP3-50230]
Standard Curve of Caspase 3/7 Activity Assay Kit (Colorimetric) [NBP3-50230]Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Caspase 3/7
Alternate Names
CASP-3, CASP-7, caspase-3, caspase-7, CPP32, CPP32B, ICE-like apoptotic protease 3, SCA-1
Additional Caspase 3/7 Products
Product Documents for Caspase 3/7 Activity Assay Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Caspase 3/7 Activity Assay Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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