CCCP

Tocris Bioscience | Catalog # 0452

Oxidative phosphorylation uncoupler
Tocris Bioscience

Key Product Details

Description

Oxidative phosphorylation uncoupler
View all Oxidative Phosphorylation Uncouplers »

Product Description

CCCP is a protonophore. It disrupts mitochondria membrane potential by transporting protons across the mitochondrial inner membrane, which interferes with the proton gradient and ATP synthesis. CCCP is a widely used as an uncoupler of oxidative phosphorylation to induce mitochondrial depolarization.

Product Specifications for CCCP

Molecular Weight

204.62

Formula

C9H5ClN4

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

Carbonyl cyanide 3-chlorophenylhydrazone

CAS Number

555-60-2

PubChem ID

2603

InChI Key

UGTJLJZQQFGTJD-UHFFFAOYSA-N

SMILES

ClC1=CC=CC(N/N=C(C#N)\C#N)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 20.46 100

Preparing Stock Solutions for CCCP

The following data is based on the product molecular weight 204.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.89 mL 24.44 mL 48.87 mL
5 mM 0.98 mL 4.89 mL 9.77 mL
10 mM 0.49 mL 2.44 mL 4.89 mL
50 mM 0.10 mL 0.49 mL 0.98 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 34 publications citing the usage of this product.

Product Documents for CCCP

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCCP

For research use only

Customer Reviews for CCCP (1)

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  • Mitochondrial studies in live neurons
    Name: Karthik krishnamurthy
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: spinal cord neurons
    Verified Customer | Posted 10/24/2018
    Time lapse images of TMRM fluorescence of mitochondria rich regions in neurons was recorded. The fluorescence intensity (F) at a given time point was normalized to the initial fluorescence intensity (F0) before addition of the AMPA. At the end of each experiment CCCP (750 nM) was added to the bath. CCCP collapses the mitochondrial membrane potential and decreases the TMRM fluorescence.
    TMRM fluorescence intensity before and after CCCP addition was used for the quantitation of change in mitochondrial membrane potential in spinal cord neurons induced by glutamate receptor agonist AMPA
    CCCP 0452

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