CHAF1A Antibody (1T1O5)

Novus Biologicals | Catalog # NBP3-16690

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1T1O5 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CHAF1A (NP_005474.2). NFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHAF1A Antibody (1T1O5)

Western Blot: CHAF1A Antibody (1T1O5) [NBP3-16690]

Western Blot: CHAF1A Antibody (1T1O5) [NBP3-16690]

Western Blot: CHAF1A Antibody (1T1O5) [NBP3-16690] - Western blot analysis of extracts of various cell lines, using CHAF1A Rabbit mAb (NBP3-16690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Applications for CHAF1A Antibody (1T1O5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHAF1A

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

CAF-1 antibodies are useful tools for cell division, DNA replication and DNA packaging.

Alternate Names

CAF, CAF1, CAF-1, CAF-1 subunit A, CAF1B, CAF1P150CAF-I 150 kDa subunit, CAF-I p150, chromatin assembly factor 1 subunit A, chromatin assembly factor 1, subunit A (p150), chromatin assembly factor I (150 kDa), Chromatin assembly factor I p150 subunit, hp150, MGC71229, P150

Gene Symbol

CHAF1A

Additional CHAF1A Products

Product Documents for CHAF1A Antibody (1T1O5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CHAF1A Antibody (1T1O5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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