Chk1 [p Ser280] Antibody (JE47-66)

Novus Biologicals | Catalog # NBP3-32754

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE47-66 expressed in HEK293
View all Chk1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser280 of human Chk1. (Uniprot: O14757)

Modification

p Ser 280

Localization

Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk1 [p Ser280] Antibody (JE47-66)

Chk1 [p Ser280] Antibody (JE47-66) Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754]

Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754]

Western blot analysis on different lysates with NBP3-32754 at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32754 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Chk1 [p Ser280] Antibody (JE47-66) Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754]

Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754]

Western blot analysis on different lysates with NBP3-32754 at 1/1,000 dilution. Lane 1: PC-12 whole cell lysate Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with lambda pp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32754 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Chk1 [p Ser280] Antibody (JE47-66)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chk1

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Chk1 [p Ser280] Antibody (JE47-66)

Certificate of Analysis

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Product Specific Notices for Chk1 [p Ser280] Antibody (JE47-66)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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