Recombinant Human Citidine Deaminase His Protein

Name: Recombinant Human Citidine Deaminase His Protein
Brand: Novus Biologicals
SKU: NBP1-49306
Price: 349 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-49306

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE

View all Citidine Deaminase Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-146 of Human Citidine Deaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Citidine Deaminase His Protein

SDS-PAGE: Recombinant Human Citidine Deaminase His Protein [NBP1-49306]

SDS-Page: Recombinant Human Citidine Deaminase Protein [NBP1-49306] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-49306

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 2 mM EDTA, 100 mM NaCl, 40% Glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Citidine Deaminase

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Cytidine deaminase

Alternate Names

CDA, CDD, EC 3.5.4.5

Gene Symbol

CDA

Additional Citidine Deaminase Products

Product Documents for Recombinant Human Citidine Deaminase His Protein

Download Product Datasheets

Download SDS-PAGE Protocol

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human Citidine Deaminase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Customer Reviews for Recombinant Human Citidine Deaminase His Protein

There are currently no reviews for this product. Be the first to review Recombinant Human Citidine Deaminase His Protein and earn rewards!

Have you used Recombinant Human Citidine Deaminase His Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image