ClariTSA™ Fluorophore Kit 620
Tocris Bioscience | Catalog # 9022
Key Product Details
Description
Product Description
ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 591 nm; emission maximum = 615 nm
Please see the product datasheet or protocol for information on product use.
ClariTSA™ is a trademark of Bio-Techne Corp.
Scientific Data Images for ClariTSA™ Fluorophore Kit 620
Application of ClariTSA™ Fluorophore Kit 620 in RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells.
The target visualised in the image is PPIB (orange, 1:1500 dilution, 620 channel, ClariTSA™ 620 kit); DAPI (dark blue, Cat. No. 5748) was used as a nuclear counterstain. Imaging was conducted using an Akoya PhenoImager with standardised imaging conditions.
Application of ClariTSA™ Fluorophore Kit 620 in RNAscope™ Multiplex Fluorescent v2 Assay in human colon tissue.
The target visualised in the image is PPIB (orange, 1:1500 dilution, 620 channel, ClariTSA™ 620 kit); DAPI (dark blue, Cat. No. 5748) was used as a nuclear counterstain. Imaging was conducted using an Akoya PhenoImager with standardised imaging conditions.Spectra Viewer
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Product Specifications for ClariTSA™ Fluorophore Kit 620
Storage
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
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Product Documents for ClariTSA™ Fluorophore Kit 620
Certificate of Analysis
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Product Specific Notices for ClariTSA™ Fluorophore Kit 620
For research use only
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