CLOCK Antibody - BSA Free

Western Blot: CLOCK Antibody [NB100-126]

Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.

Western Blot: CLOCK Antibody - BSA Free [NB100-126] -

Circadian gene expression varies between pHGGs and pLGGs. (A) Interleaved scatter plots represent bulk RNA sequencing (rsem FPKM) analysis comparing circadian gene expression across pediatric low-grade glioma (pLGG, n = 426, blue) and pediatric high-grade glioma (pHGG, n = 211, red) samples from the Pediatric Brain Tumor Atlas (PBTA, Provisional). Data are expressed as mean +/- SEM, and expression values are log₂-transformed FPKM counts. Statistical analysis was performed using a two-way ANOVA with Benjamini and Hochberg false discovery rate (FDR) to determine differences in gene expression between pHGGs and pLGGs. Each dot represents an individual tumor sample. Asterisks indicate statistically significant differences (*P < 0.05), while “NS" denotes non-significant comparisons. (B) Western blots of four representative pediatric high-grade glioma tissue samples (left) and four pediatric low-grade glioma tissue samples (right) comparing BMAL1, CLOCK, PER2, and REV-ERB alpha protein expression (sections of separate blots displayed; full blots are shown in Supplementary Figure S3). Of note, tissue sample HGG 3 is derived from the same patient as the STN-49 cell line. (C) Normalization to beta -ACTIN control reveals BMAL1 (p < 0.05, 1.57 vs. 0.98) and CLOCK (p < 0.05, 0.98 vs. 0.39) protein expression is higher in high-grade gliomas compared to low-grade gliomas, REV-ERB alpha is lower (p < 0.05, 0.12 vs. 0.55), and there are no significant differences in PER2 protein expression. Data are expressed as mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-025-17461-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.