Complement Factor H Antibody (OTI5H5)

Novus Biologicals | Catalog # NBP2-46263

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5H5
View all Complement Factor H Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 125-346 of human CFH (NP_001014975) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Complement Factor H Antibody (OTI5H5) (NBP2-46263) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Complement Factor H Antibody (OTI5H5)

Western Blot: Complement Factor H Antibody (OTI5H5) [NBP2-46263]

Western Blot: Complement Factor H Antibody (OTI5H5) [NBP2-46263]

Western Blot: Complement Factor H Antibody (5H5) [NBP2-46263] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Complement factor H.

Applications for Complement Factor H Antibody (OTI5H5)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Complement Factor H

The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy. Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600mg/L for the 155 kDa form and 1-5mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.

Alternate Names

ARMD4, ARMS1, beta-1-H-globulin, CFH, CFHL3, FH, FHL1, HF1, HF2, HUS

Gene Symbol

CFH

Additional Complement Factor H Products

Product Documents for Complement Factor H Antibody (OTI5H5)

Certificate of Analysis

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Product Specific Notices for Complement Factor H Antibody (OTI5H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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