CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-03233
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Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human CXCL2/GRO beta/MIP-2/CINC-3 (NP_002080.1). MARATLSAAPSNPRLLRVALLLLLLVAASRRAAGAPLATELRCQCLQTLQGIHLKNIQSVKVKSPGPHCAQTEVIATLKNGQKACLNPASPMVKKIIEKMLKNGKSN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free (NBP3-03233) is a polyclonal antibody validated for use in IHC and WB. Anti-CXCL2/GRO beta/MIP-2/CINC-3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
Western Blot: CXCL2/GRO beta/MIP-2/CINC-3 AntibodyAzide and BSA Free [NBP3-03233]
Western Blot: CXCL2/GRO beta/MIP-2/CINC-3 Antibody [NBP3-03233] - Analysis of extracts of rat lung, using CxCL2/GRO beta/MIP-2/CINC-3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Applications for CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Alternate Names
CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2
Gene Symbol
CXCL2
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
Product Specific Notices for CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.govRelated Research Areas
Citations for CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CXCL2/GRO beta/MIP-2/CINC-3 Antibody - Azide and BSA Free
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A: Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.
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