Cyclopiazonic acid

Tocris Bioscience | Catalog # 1235

Inhibitor of SERCA ATPase
Tocris Bioscience

Key Product Details

Description

Inhibitor of SERCA ATPase

Alternative Names

α-Cyclopiazonic acid
View all Ca2+-ATPase Inhibitors »

Product Description

Cyclopiazonic acid is a cell-permeable, reversible inhibitor of sarcoplasmic reticulum Ca2+-ATPase.

Product Specifications for Cyclopiazonic acid

Molecular Weight

336.39

Formula

C20H20N2O3

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(6aR,11aS,11bR)-rel-10-Acetyl-2,6,6a,7,11a,11b-hexahydro-7,7-dimethyl-9H-pyrrolo[1',2':2,3]isoindolo[4,5,6-cd]indol-9-one

CAS Number

18172-33-3

PubChem ID

54682463

InChI Key

SZINUGQCTHLQAZ-DQYPLSBCSA-N

SMILES

OC([C@](N4C(C)5C)([H])[C@]1([H])[C@@]5([H])CC3=C2C1=CNC2=CC=C3)=C(C(C)=O)C4=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.64 100

Preparing Stock Solutions for Cyclopiazonic acid

The following data is based on the product molecular weight 336.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.97 mL 14.86 mL 29.73 mL
5 mM 0.59 mL 2.97 mL 5.95 mL
10 mM 0.30 mL 1.49 mL 2.97 mL
50 mM 0.06 mL 0.30 mL 0.59 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 149 publications citing the usage of this product.

Product Documents for Cyclopiazonic acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cyclopiazonic acid

For research use only

Related Research Areas

Ion Channels and Regulators

Citations for Cyclopiazonic acid

Customer Reviews for Cyclopiazonic acid (2)

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  • Cyclopiazonic Acid Blocks Re-Uptake of Ca2+ Into ER leading to increased T-cell activation by IFNg
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Primary Cd8+ T-cells
    Verified Customer | Posted 01/23/2019
    Cyclopiazonic acid (CPA) was used to block SERCA in activated CD8+ T-cells. This maintained high intracellular Ca2+, leading to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.
    Cyclopiazonic acid 1235
  • Cyclopiazonic acid (CPA) depletes ER Ca2+ stores, leading to STIM1 clustering in in HEK293 cells.
    Name: Niroj Shrestha
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 11/02/2018
    ER resident protein, STIM1 was expressed with YFP fluorescent tag in HEK293 cells and imaged using TIRF microscopy. STIM1 (left) clusters to form punctae at PM-ER junctions upon ER Ca2+ store depletion (right) by CPA (25 µM in DMSO), thereby activating Orai1-mediated Ca2+ entry.
    Cyclopiazonic acid 1235

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