Cytochrome P450 17A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16077

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Monkey (91%), Primate (99%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytochrome P450 17A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytochrome P450 17A1. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (91%), Chimpanzee (99%).

Localization

Membrane; Single-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 17A1 Antibody - BSA Free

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077]

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077]

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.
Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077]

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077]

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077]

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077]

Western Blot: Cytochrome P450 17A1 Antibody [NBP2-16077] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYP17A1 antibody [N1C2] diluted at 1:1000.

Applications for Cytochrome P450 17A1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 17A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq]

Long Name

Steroid 17-alpha-hydroxylase/17,20 lyase

Alternate Names

CYP17, CYP17A1, CYPXVII, Cytochrome P450-C17, Cytochrome P450c17, EC 1.14.14.19, EC 1.14.14.32, S17AH

Gene Symbol

CYP17A1

UniProt

P05093 (Human)

Additional Cytochrome P450 17A1 Products

Product Documents for Cytochrome P450 17A1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytochrome P450 17A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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