DBCO PEG4 Biotin

Key Product Details

Product Description

DBCO-PEG4-Biotin is a biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction. The hydrophilic PEG4 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. The PEG linker in DBCO-PEG4-Biotin also enhances the accessibility of the biotin moiety, improving the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.

Product Specifications for DBCO PEG4 Biotin

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for DBCO PEG4 Biotin

The following data is based on the product molecular weight 749.92.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.33 mL	6.67 mL	13.33 mL
5 mM	0.27 mL	1.33 mL	2.67 mL
10 mM	0.13 mL	0.67 mL	1.33 mL
50 mM	0.03 mL	0.13 mL	0.27 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Sawant A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acids Res. 2016 PMID: 26384420
• Bernardim Precise installation of diazo-tagged side-chains on proteins to enable in vitro and in-cell site-specific labeling. Bioconjug.Chem. 2020 PMID: 32375474
• Darabedian O-Acetylated chemical reporters of glycosylation can display metabolism-dependent background labeling of proteins but are generally reliable tools for the identification of glycoproteins. Front.Chem. 2020 PMID: 32411667
• Agard A comparative study of bioorthogonal reactions with azides. ACS Chem.Biol. 2006 PMID: 17175580

Product Documents for DBCO PEG4 Biotin