DFHBI

Key Product Details

Product Description

DFHBI is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.

Product Specifications for DFHBI

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for DFHBI

The following data is based on the product molecular weight 252.22.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.96 mL	19.82 mL	39.65 mL
5 mM	0.79 mL	3.96 mL	7.93 mL
10 mM	0.40 mL	1.98 mL	3.96 mL
50 mM	0.08 mL	0.40 mL	0.79 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.

• Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
• Svensen and Jaffrey Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 2016 PMID: 26877022
• Paige RNA mimics of green fluorescent protein. Science 2011 PMID: 21798953
• Strack Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat.Protoc. 2014 PMID: 24356773
• Filonov In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 2015 PMID: 26000751
• Song Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 2014 PMID: 24393009

Product Documents for DFHBI