DFHO

Key Product Details

Product Description

DFHO is a mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. DFHO fluorescence is activated by binding to Corn aptamer (K_d = 70 nM) or Squash aptamer (K_d = 54 nM). Excitation/emission maxima (λ) = 505/545 nm; extinction coefficient = 29000 M^-1 cm^-1.

Product Specifications for DFHO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for DFHO

The following data is based on the product molecular weight 281.22.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.56 mL	17.78 mL	35.56 mL
5 mM	0.71 mL	3.56 mL	7.11 mL
10 mM	0.36 mL	1.78 mL	3.56 mL
50 mM	0.07 mL	0.36 mL	0.71 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 5 publications citing the usage of this product.

• Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
• Warner A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat.Chem.Biol. 2017 PMID: 28945234
• Dey Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 2022 PMID: 34937909
• Song Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat.Chem.Biol. 2017 PMID: 28945233

Product Documents for DFHO