DMHBO+
Tocris Bioscience | Catalog # 7764
Key Product Details
Description
Wavelength
Product Description
DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.Optical Data
| λabs | 456 nm |
| λem | 592 nm |
| Quantum Yield (φ) | 0.1 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
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Product Specifications for DMHBO+
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 27.62 | 50 |
Preparing Stock Solutions for DMHBO+
The following data is based on the product molecular weight 552.37.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.62 mL | 18.10 mL | 36.21 mL |
| 2.5 mM | 0.72 mL | 3.62 mL | 7.24 mL |
| 5 mM | 0.36 mL | 1.81 mL | 3.62 mL |
| 25 mM | 0.07 mL | 0.36 mL | 0.72 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Steinmetzger A multicolor large stokes shift fluorogen-activating RNA aptamer with cationic chromophores. Chemistry 2019 PMID: 30485561
Product Documents for DMHBO+
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DMHBO+
For research use only
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