DNMT1 Activity/Inhibition Assay Kit (Colorimetric)
Novus Biologicals | Catalog # KA1515
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Key Product Details
Sample Type & Volume Required Per Well
Nuclear Extract, Purified Enzyme (1-5 uL (5 to 10 ug nuclear extract or 10 to 100 ng purified enzyme included))
Sensitivity
0.5 ug nuclear extract or 0.5 ng purified enzymes
Assay Range
0.5 to 20 ug nuclear extract, 0.5 to 200 ng purified enzyme
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Product Specifications
Assay Type
Colorimetric
Kit Type
Activity/Inhibition Assay Kit (Colorimetric)
Species
Mouse
Description
Quality control test: Standard Curve DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric) is use for screening DNMT inhibitors.
Scientific Data Images for DNMT1 Activity/Inhibition Assay Kit (Colorimetric)
DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - Positive control by using nuclear extract from MCF-7 cells.
Kit Contents for DNMT1 Activity/Inhibition Assay Kit (Colorimetric)
- 8-Well Assay Strips (With Frame)
- Adhesive Covering Film
- MU1 (10X Wash Buffer)
- MU2 (DNMT Assay Buffer)
- MU3 (Adomet, 50X)*
- MU4 (DNMT Enzyme Control, 50 µg/mL)*
- MU5 (Capture Antibody, 1000 µg/mL*)
- MU6 (Detection Antibody, 400 µg/mL)*
- MU7 (Enhancer Solution)*
- MU8 (Developer Solution)
- MU9 (Stop Solution)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: DNMT1
Long Name
DNA [Cytosine-5-]-Methyltransferase 1
Alternate Names
CXXC9, MCMT
Gene Symbol
DNMT1
Additional DNMT1 Products
Product Documents for DNMT1 Activity/Inhibition Assay Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DNMT1 Activity/Inhibition Assay Kit (Colorimetric)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
Citations for DNMT1 Activity/Inhibition Assay Kit (Colorimetric)
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