DOCK2 Antibody (JE53-62)

Novus Biologicals | Catalog # NBP3-32276

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE53-62 expressed in HEK293
View all DOCK2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human DOCK2 aa 1,781-1,830 / 1,830. (Uniprot: Q92608)

Localization

Endomembrane system, Cytoplasm, cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

212 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit DOCK2 Antibody (JE53-62) (NBP3-32276) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DOCK2 Antibody (JE53-62)

DOCK2 Antibody (JE53-62)

Western Blot: DOCK2 Antibody (JE53-62) [NBP3-32276]

Western Blot: DOCK2 Antibody (JE53-62) [NBP3-32276] - Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (NBP3-32276) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Raji cell lysate
Lane 4: 293T cell lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 212 kDa
Observed band size: 180 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32276) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for DOCK2 Antibody (JE53-62)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DOCK2

The DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocytechemotaxis.(supplied by OMIM)

Long Name

Dedicator of Cytokinesis Protein 2

Alternate Names

dedicator of cytokinesis 2, dedicator of cytokinesis protein 2, FLJ46592, KIAA0209dedicator of cyto-kinesis 2

Gene Symbol

DOCK2

Additional DOCK2 Products

Product Documents for DOCK2 Antibody (JE53-62)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DOCK2 Antibody (JE53-62)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Natural Killer T (NKT) Cells

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