Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase His Protein

Novus Biologicals | Catalog # NBP2-75954

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all Double-Stranded Uracil-DNA Glycosylase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-168 of E.coli Double-Stranded Uracil-DNA Glycosylase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images

SDS-PAGE: Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase His Protein [NBP2-75954]

SDS-PAGE: Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase His Protein [NBP2-75954]

SDS-Page: Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase Protein [NBP2-75954] - Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase Protein

Formulation, Preparation, and Storage

NBP2-75954
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Double-Stranded Uracil-DNA Glycosylase

G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

double-strand uracil-DNA glycosylase, Double-strand-specific uracil glycosylase, dsDNA specific UDG, DsUDG, EC 3.2.2.28, G/U mismatch-specific DNA glycosylase, G:T/U mismatch-specific DNA glycosylase, Mismatch-specific uracil DNA glycosylase, Mug, Xanthine DNA glycosylase

Additional Double-Stranded Uracil-DNA Glycosylase Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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