Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all DPH4 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DPH4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DPH4 Antibody - Azide and BSA Free

Western Blot: DPH4 Antibody [NBP2-99139]

Western Blot: DPH4 Antibody [NBP2-99139]

Western Blot: DPH4 Antibody [NBP2-99139] - Anti-DPH4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 26 kDa
Immunoprecipitation: DPH4 Antibody [NBP2-99139]

Immunoprecipitation: DPH4 Antibody [NBP2-99139]

Immunoprecipitation: DPH4 Antibody [NBP2-99139] - DPH4 was immunoprecipitated using: Lane A: 0.5 mg HL-60 Whole Cell Lysate. Lane B: 0.5 mg Raw264.7 Whole Cell Lysate4 ul anti-DPH4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DPH4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 26 kDa

Applications for DPH4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPH4

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylationand inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved insynthesis of diphthamide in EEF2 (Liu et al., 2004 (PubMed 15485916)).(supplied by OMIM)

Alternate Names

CSL-type zinc finger-containing protein 3, DnaJ (Hsp40) homolog, subfamily C, member 24, dnaJ homolog subfamily C member 24, DPH4 homolog, DPH4, JJJ3 homolog, DPH4, JJJ3 homolog (S. cerevisiae), DPH41700030A21Rik, JJJ3, S. cerevisiae), ZCSL3, zinc finger, CSL domain containing 3, zinc finger, CSL-type containing 3

Gene Symbol

DNAJC24

Additional DPH4 Products

Product Documents for DPH4 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DPH4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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