Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DUSP12 Primary Antibodies »

Product Specifications

Immunogen

Recognizes region between residues Between Between 1 and 50..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DUSP12 Antibody - BSA Free

DUSP12 Antibody

Western Blot-DUSP12 Antibody-NBP3-18420-

Western Blot-DUSP12 Antibody-NBP3-18420-Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITPNB antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
DUSP12 Antibody

Immunoprecipitation-DUSP12 Antibody-NBP3-18420-

Immunoprecipitation-DUSP12 Antibody-NBP3-18420-. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DUSP12 antibody used for IP at 2 ug per reaction. DUSP12 was also immunoprecipitated by rabbit anti-DUSP12 antibody. For blotting immunoprecipitated DUSP12, was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
DUSP12 Antibody

Western Blot;DUSP12 Antibody[NBP3-18420]

Western Blot;DUSP12 Antibody Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DUSP12 antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DUSP12 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

20 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DUSP12

DUSP12 is encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product is the human ortholog of the Saccharomyces cerevisiae YVH1 protein tyrosine phosphatase. It is localized predominantly in the nucleus, and is novel in that it contains, and is regulated by a zinc finger domain.

Alternate Names

dual specificity phosphatase 12, dual specificity protein phosphatase 12, Dual specificity tyrosine phosphatase YVH1, DUSP1YVH1 protein-tyrosine phosphatase ortholog, EC 3.1.3.48, serine/threonine specific protein phosphatase, TDSP4, YVH1EC 3.1.3.16

Gene Symbol

DUSP12

Additional DUSP12 Products

Product Documents for DUSP12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DUSP12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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