E 64

Tocris Bioscience | Catalog # 5208

Potent and irreversible cysteine protease inhibitor
Tocris Bioscience

Key Product Details

Description

Potent and irreversible cysteine protease inhibitor
View all Synthetic Cathepsin Inhibitors »

Product Description

E 64 is a potent and irreversible cysteine protease inhibitor (IC50 values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.

Product Specifications for E 64

Molecular Weight

357.41

Formula

C15H27N5O5

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(2S,3S)-3-[[[(1S)-1-[[[4-(Aminoiminomethyl)amino]butyl]amino]carbonyl]-3-methylbutyl]amino]carbonyl]-2-oxiranecarboxylic acid

CAS Number

66701-25-5

PubChem ID

123985

InChI Key

LTLYEAJONXGNFG-DCAQKATOSA-N

SMILES

O=[C@@](N[C@H]([C@](NCCCCNC(N)=N)=O)CC(C)C)[C@@H]1[C@@H](C(O)=O)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 7.15 20

Preparing Stock Solutions for E 64

The following data is based on the product molecular weight 357.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 13.99 mL 69.95 mL 139.90 mL
1 mM 2.80 mL 13.99 mL 27.98 mL
2 mM 1.40 mL 6.99 mL 13.99 mL
10 mM 0.28 mL 1.40 mL 2.80 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 14 publications citing the usage of this product.

Product Documents for E 64

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for E 64

For research use only

Customer Reviews for E 64 (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used E 64?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Reduction of cathepsin acitivity in vitro with E64 inhibitor
    Name: Yann Malato
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: ARPE19
    Verified Customer | Posted 10/03/2024
    I used the E64 in ARPE19 cells after sodium iodate administration with a fluorescent cathepsin sensor (magic red). I used E64 at 10uM and it reduced the signal of magic red vs DMSO 0.01% control

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All