EDA-DA

Tocris Bioscience | Catalog # 7714

Unnatural dipeptide building block with alkyne group for functionalizing peptidoglycan
Tocris Bioscience

Key Product Details

Description

Unnatural dipeptide building block with alkyne group for functionalizing peptidoglycan
View all Unnatural Amino Acids »

Product Description

EDA-DA is an unnatural dipeptide building block (an ethynyl-D-alanine and a D-alanine). It incorporates a biorthogonal alkyne group into peptidoglycan (PG) through MurF in the cytoplasmic pathway, which enables selective labeling via a click-chemistry reaction. EDA-DA allows labeling of PG in Gram-positive (B. subtilis), Gram-negative (E. coli and C. trachomatis), Mycobacterium (M. smegmatis) and moss plastids (P. patens) with azide modified fluorescent dyes such as Alexa Fluor 488.

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Product Specifications for EDA-DA

Molecular Weight

298.22

Formula

C8H12N2O3.CF3CO2H

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-(4,4,5,5-Tetradehydro-D-norvalyl)-D-alanine trifluoroacetate

CAS Number

87156-01-2

InChI Key

BRIXKPONTJDKNO-KGZKBUQUSA-N

SMILES

C[C@H](C(O)=O)NC([C@@H](CC#C)N)=O.FC(F)(F)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 29.82 100
DMSO 29.82 100

Preparing Stock Solutions for EDA-DA

The following data is based on the product molecular weight 298.22.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.35 mL 16.77 mL 33.53 mL
5 mM 0.67 mL 3.35 mL 6.71 mL
10 mM 0.34 mL 1.68 mL 3.35 mL
50 mM 0.07 mL 0.34 mL 0.67 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for EDA-DA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EDA-DA

For research use only

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