EDC4 Antibody

Novus Biologicals | Catalog # NB100-2273

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all EDC4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human RCD8 Autoantigen using the numbering given in entry AAH43616 (GeneID 23644).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EDC4 Antibody

Western Blot: EDC4 Antibody [NB100-2273]

Western Blot: EDC4 Antibody [NB100-2273]

Western Blot: EDC4 Antibody [NB100-2273] - Detection of mouse RCD8 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCD8 antibody NB100-2273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: EDC4 Antibody [NB100-2273]

Western Blot: EDC4 Antibody [NB100-2273]

Western Blot: EDC4 Antibody [NB100-2273] - Detection of human RCD8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCD8 antibody NB100-2273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: EDC4 Antibody [NB100-2273]

Immunoprecipitation: EDC4 Antibody [NB100-2273]

Immunoprecipitation: EDC4 Antibody [NB100-2273] - Detection of human RCD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RCD8 antibody NB100-2273 (lot NB100-2273-2) used for IP at 6 ug per reaction. RCD8 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2273-1). For blotting immunoprecipitated RCD8, A300-745A was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for EDC4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EDC4

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro)

Alternate Names

Autoantigen Ge-1, Autoantigen RCD-8, enhancer of mRNA decapping 4, enhancer of mRNA-decapping protein 4, Ge-1, Hedls, HEDLSHuman enhancer of decapping large subunit, RCD-8

Entrez Gene IDs

23644 (Human); 234699 (Mouse)

Gene Symbol

EDC4

UniProt

Q6P2E9 (Human)

Additional EDC4 Products

Product Documents for EDC4 Antibody

Certificate of Analysis

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Product Specific Notices for EDC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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