EI2BL Overexpression Lysate

Name: EI2BL Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-13065
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-13065

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for EI2BL Overexpression Lysate

EI2BL Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC200993

Accession#: NM_001031727

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for EI2BL Overexpression Lysate

Western Blot: EI2BL Overexpression Lysate [NBL1-13065]

Western Blot: EI2BL Overexpression Lysate (Adult Normal) [NBL1-13065] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EI2BL.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: EI2BL

Alternate Names

EC 5.3.1.23, M1Pi, Mediator of RhoA-dependent invasion, methylthioribose-1-phosphate isomerase, methylthioribose-1-phosphate isomerase homolog (S. cerevisiae), MGC3207, MRDI, MTNA, MTR-1-P isomerase, S-methyl-5-thioribose-1-phosphate isomerase, Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein, Ypr118w

Gene Symbol

MRI1

Additional EI2BL Products

Product Documents for EI2BL Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for EI2BL Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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