eIFs are heterotrimeric initiation complexes that form on the ribosome during protein synthesis. Each eIF serves a distinct function during protein synthesis to help attach, assemble, and drive the ribosome along the mRNA.
eIF2 alpha/EIF2S1 [p Ser52] Antibody (849159) [FITC]
Novus Biologicals | Catalog # FAB39971F
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 849159
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Product Specifications
Immunogen
Phosphopeptide containing the human eIF2 alpha S52 site
Accession # P05198
Accession # P05198
Modification
p Ser52
Specificity
Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for eIF2 alpha/EIF2S1 [p Ser52] Antibody (849159) [FITC]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB39971. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: eIF2 alpha
Long Name
Eukaryotic Translation Initiation Factor 2 Alpha
Alternate Names
EIF2S1
Gene Symbol
EIF2S1
Additional eIF2 alpha Products
Product Documents for eIF2 alpha/EIF2S1 [p Ser52] Antibody (849159) [FITC]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for eIF2 alpha/EIF2S1 [p Ser52] Antibody (849159) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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