Endotoxin End-Point Quantitation Kit

INTENDED USE AND DESCRIPTION

The R&D Systems™ End-Point Endotoxin Quantitation Assay provides a highly sensitive and reliable in vitro method for accurately detecting and quantifying endotoxin levels in biological samples. The kit contains a Lyophilized Amebocyte Lysate (AL) Reagent, derived from circulating amebocytes of the Asian horseshoe crab Tachypleus tridentatus. When endotoxins are present in a sample, they activate proenzymes in the lysate, leading to the formation of coagulase enzymes. These enzymes catalyze the hydrolysis of a colorless substrate, resulting in the release of the yellow chromophore p-nitroaniline (pNA). The resulting color intensity is measured quantitatively at 405 nm, showing a linear correlation between absorbance and endotoxin concentration within the ranges of 0.01-0.1 EU/mL and 0.1-1.0 EU/mL.

The Endotoxin End-Point Chromogenic Assay provides procedures for two endotoxin concentration ranges, 0.1-1.0 EU/mL and 0.01–0.1 EU/mL, determined by different incubation times. The AL Reagent is not labeled with a specific sensitivity; sensitivity is defined as the lowest endotoxin concentration used to construct the standard curve. The lowest detectable limit of this assay kit is 0.01 EU/mL.

COMPONENTS

• Lyophilized Amebocyte Lysate (AL)
• Endotoxin-free Water
• Endotoxin Control Standard
• Chromogenic Substrate